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Trial Number 6

Client Number 494

Project Number 1

Trial Purpose:

To test the gram-negative and gram-positive bacteria removal efficacy of E-Cloth products off various substrates.

Date Run:

07/13/2022

Experiment Procedure:

Gram-negative and gram-positive bacteria, Escherichia coli (E.coli) ATCC 29214 and Listeria monocytogenes (L. monocytogenes) ATCC 35752, were sub-cultured twenty-four hours in tryptic soy broth (TSB) at 37°C (98.6°F) prior to testing. For each new E-cloth product, four coupons (2" x 1") of a specified substrate were contained in a sterile petri dish to act as the positive control (P+), negative control (N-), Test 1 (T1), and Test 2 (T2) substrates. The substrates tested for each bacteria type included glass, laminate, and stainless steel related to the product use parameters designated by E-cloth. Ten microliters of the bacteria inoculated the P+, T1, and T2 coupons. Coupons were incubated at room temperature for 15 minutes in an aseptic environment before treating the N-, T1, and T2 coupons with an E-cloth product. The P+ coupons were placed into 50ml conical tubes containing Dey and Engley (DE) Neutralizing broth.

Supplied new E-cloth products were dipped in Deionized (DI) water before manually squeezing to keep the product damp before attaching to the sled of the Straight-Line Washability (SLW) unit. The SLW unit simulated manual wiping by passing three times over the contaminated coupon surface before placing the cleaned coupon immediately into each of the respective D/E broth conical tubes with sterile forceps. The conical tubes were placed on the shaker for 10 minutes before performing serial dilutions in 1X PBS up to 104 (1:1000). Stock (1:1) and serial dilution solutions were plated and spread on tryptic soy agar (TSA). Plates were incubated at 37°C overnight. Isolated colonies were counted the following day to calculate log reduction and percent removal for each disinfection test parameter performed. 

Trial Results:

Single Wipe:

Product Substrate Organism Log Reduction % Reduction
Deep Clean Mop Head Laminate E.coli 29214 1.433 96.0727
L. monocytogenes 35752 2.0932 98.5088
Deep Clean Mop Head Wood E.coli 29214 2.0251 99.0430
L. monocytogenes 35752    
Kitchen Dynamo Laminate E.coli 29214 1.9572 98.0833
L. monocytogenes 35752 1.1454 87.1596
Kitchen Cleaning Cloth Laminate E.coli 29214 2.9724 99.8785
L. monocytogenes 35752    
Non-scratch Scrubbing Pad Laminate E.coli 29214    
L. monocytogenes 35752 6.9100 100.000
Stainless Steel Cloth Stainless Steel E.coli 29214 1.1494 79.4231
L. monocytogenes 35752 0.2625 43.3094
Washing Up Pad Laminate E.coli 29214 1.7693 97.5302
L. monocytogenes 35752 1.3259 94.6183
Window Cleaning Waffle Weave Cloth Glass E.coli 29214 0.3007 41.8317
L. monocytogenes 35752 1.1028 88.6389

Multiple Wipes (three total):

Product Substrate Organism Log Reduction % Reduction
Deep Clean Mop Head Laminate E.coli 29214 2.0719 98.8341
L. monocytogenes 35752 1.6470 97.6194
Deep Clean Mop Head Wood E.coli 29214 4.5100 99.9408
L. monocytogenes 35752 2.1094 99.2097
Kitchen Dynamo Laminate E.coli 29214 2.3006 99.4757
L. monocytogenes 35752 3.0432 99.9083
Kitchen Cleaning Cloth Laminate E.coli 29214 2.7793 99.9045
L. monocytogenes 35752 2.5188 99.6971
Non-scratch Scrubbing Pad Laminate E.coli 29214 1.6264 96.9966
L. monocytogenes 35752 2.2902 99.4729
Stainless Steel Cloth Stainless Steel E.coli 29214 1.5058 94.8134
L. monocytogenes 35752 2.6469 99.7659
Washing Up Pad Laminate E.coli 29214 1.8647 98.6132
L. monocytogenes 35752 1.1209 91.8265
Window Cleaning Waffle Weave Cloth Glass E.coli 29214 1.2412 94.2493
L. monocytogenes 35752 1.4161 95.6140

Success Rating:

Results successful using TACT (time, agitation, concentration, and temperature, as well as rinsing and drying) and/or other cleaning chemistries examined.

Conclusion:

A majority of the E. cloth products produced a one to two log reduction removal of gram-negative and gram-positive bacteria on the specified glass, laminate, and stainless steel surfaces associated with each product. The Non-scratch Scrubbing Pad was the most effective after a single wipe by removing 100% of L. monocytogenes on laminate. The Kitchen Dynamo performed the best with a three-log reduction of L. monocytogenes after three manual wipes on a laminate surface. 

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