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Trial Number 0

Trial Purpose:

To train staff on how to titrate quat based disinfectant product for QC

Date Run:

04/02/2014

Experiment Procedure:

Conducted training of lab technician at Tropical on the Standard Operating Procedure for Quality Control of Quaternary Based Products. Went over Electrode Preparation, Preparation of sodium dodecyl sulfate (SDS) Titrant, Burette Preparation, ample Preparation for CONCENTRATE and RTU.

Materials

2-Propanol (isopropyl alcohol)

Deionized or Distilled water

2’,7’-Dichlorofluorescein indicator 0.1% in 2-Propanol

Glacial acetic acid

0.1N Silver Nitrate (AgNO3) solution, standardized

0.1N Sodium Hydroxide (NaOH) solution, standardized (for Amine Hydrochloride determination)

Phenophthalein indicator, 1% in 2-Propanol (for Amine Hydrochloride determination)

 

2 burets, 1 for AgNO3 and 1 for NaOH

 

Procedure

Make a 1:1 solution of 2-Propanol and Deionized or Distilled water

Accurately weigh to 0.001g of sample into a 250 mL Ehrlenmeyer flask

Add 50mL of the 1:1 solution of 2-Propanol and Deionized or Distilled water

Add 6 drops of 0.1% 2’,7’-Dichlorofluorescein indicator

Add 1-2 drops of Glacial acetic acid, or amount sufficient to remove the fluorescence

Titrate with 0.1N AgNO3 to the pink end-point

 

 

Procedure for Amine Hydrochloride (Amine HCl) determination

Accurately weigh to 0.001g of sample into a 250 mL Ehrlenmeyer flask

Add 50mL of Deionized or Distilled water

Add 6 drops of 1% Phenophthalein indicator

Titrate with 0.1N NaOH to the pink end-point

Success Rating:

A cleanliness study, addressing only various analytical techniques.

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